Low-level analysis can be very difficult. Basic issues in both qualitative and quantitative analyses will be described.
Trace-level analysis under Good Laboratory Practices or ISO 17025 is quite different when the target analytes are at very low amounts. The effect and requirements for accuracy, precision, selectivity, and other validation requirements are demanded are different than and more difficult to meet than those for higher concentrations. Basic parameters, such as precision and accuracy, are more difficult. Qualitative (identification of a molecule’s structure and identity) can be much more difficult.